Simmons citrate agar
Thanks to the Simmon's agar mineral composition its meets the needs of the microorganisms. But the only source of carbon in the environment is citrate in the form of sodium salt. Due to this environment favors the growth of citrate-positive microorganisms. During their development the waste products raise pH, is manifested by a color change indicator (bronchiology blue) from green to blue. The analysis is conducted within 72 hours, although preliminary results can already be seen in a day.
Medium Olkenitskyi
Medium Olkenitskyi, also known as three-sugar agar with urea. The sterile molten medium was poured into vials so that when cold formed filled "style" and "school" in contact with the air. The color of the finished medium is red or orange. The sowing is carried out by puncturing the environment of the microbial loop to the bottom of the tube. Samples cultured at 37 ° C for 24 hours, after which evaluate the change in the appearance of the environment. It can be used to identify the 5 indicators:
Fermentation of glucose - yellow "column";
Fermentation of sucrose and / or lactose - yellow "school";
The formation of hydrogen sulphite - blackening;
Fermentation of urea - crimson color;
The formation of gas - bubbles in the thickness of the agar.
Medium Bleeckfeldt's
The broth for the accumulation of lactic acid bacteria and identification of their growth. Liquid nutrient medium is characterized by a high growth quality, thereby contributes to the rapid development of lactic acid bacteria. Inhibitors and selective agents are absent, so clear culture is necessary to use. The indicator bromocresol purple reveals the formation of acids (metabolic products of the bacteria) by the color change from purple to yellow. The time of culturing 48 hours at 37 °C.
Lactobacagar
Lactobacagar - the differential nutrient medium that stimulates the growth of lactobacilli and inhibits extraneous microflora. The nutritional part of the environment consists of complex organic components (peptone, yeast extract), carbohydrate (glucose) and mineral supplements (magnesium, manganese). The precise composition optimal provides "picky" Lactobacillus nutrients in the required concentrations. When the salts of organic acids (acetate, citrate), and low pH inhibit the growth of extraneous microorganisms. This makes it possible to determine the presence of Lactobacillus in such planted samples as dairy and foods, or defecation. Samples cultured at 37 ° C for 48 hours to hold dishes with gasparatos providing the concentration of carbon dioxide 5-10%.
