Sabouraud agar
Prepared nutrient medium in the composition is 4% glucose, optimal for the development of fungi and yeast. Peptone is a source of nitrogenous substances, and yeast extract intensificare microorganisms to growth by ensuring complex organic compounds. In addition to the "Sabouraud agar" we produce "Sabouraud broth", which is similar but has a liquid consistency.
For convenience, we also produce a modification of the medium with the addition of chloramphenicol, which inhibits the growth of bacteria. This is useful in particular for the analysis of resistance disco-diffuse method.
Table №1. Sabouraud agar
Table №2. With chloramphenicol
Acetate agar
Acetate agar is used for differentiation according to the ability to ferment acetate. Due to the fact that acetate in the medium composition is the sole source of carbon, bacterial growth, are not capable of disposal, is suppressed. Bacteria that fermented it in the course of their life produce an alkaline solution which changes the medium color from green to blue. Samples cultured at 37 ° C, the final result is evaluated after 72 hours, although usually within 24 hours the reaction is already taking place. The duration of cultivation depends on the strain that you analyze.
Glucose-peptone medium
Liquid nutrient medium for the accumulation of enterobacteria. The qualitative composition of the medium is a nutrient base for the growth of bacteria by providing them with all the necessary nutrients as a carbon source included glucose, allows you to store a wide range of microorganisms. Their development in addition to opacity also easy to detect a change in the medium from green to yellow, and using the glass "float" to detect the formation of gas.
Nutrient medium Kessler
Kessler's nutrient medium is used to identify the Escherichia coli in the sample. This is achieved through the two factors of the nutrient medium. The presence of bile inhibits related nonpathogenic microflora, crystal violet inhibits gram-positive bacteria, and lactose as the sole source of carbon inhibits bacteria that cannot ferment lactose. Applying the glass "float" allows to visually detect the presence of coliforms on the basis of the evolution of gas.
