Corynebacagar

The differential medium that is used for the production of Corynebacterium diphtheria. To stimulate the growth of Corynebacterium necessary blood components or blood substitutes. To inhibit the growth of concomitant microflora to the environment is added a solution of potassium tellurite. Also, corynebacteria, growing on terricolous environments recover metallic tellurium from tellurite potassium and changes the color of the colony to black. BioWare Corynebacterium is distinguished by the form of the colonies:

• Corynebacterium diphtheriae gravis - dark grey round slightly flattened colonies with a diameter of 2 - 3 mm. Under a microscope (25x) colonies with slightly irregular edges and folded surface (a type of "Daisy").
• Corynebacterium diphtheriae mitis - dark grey round convex shiny colonies with smooth edges, with a diameter of 1.5 - 3 mm.
• Corynebacterium xerosis - dark grey round convex shiny colonies with smooth edges, a diameter of 1.2 - 2 mm.
• Corynebacterium ulcerans - dark grey round convex shiny colonies with smooth edges, a diameter of 0.5 - 1.5 mm with a silver rim.

Baird Parker agar base

This agar is used for isolation and quantification of staphylococci. The composition includes tellurite solution of potassium and lithium chloride, which are growth inhibitors concomitant microflora. Also, staph recovers tellurium from tellurite potassium, which stains its colonies in the black color. Glycine and sodium pyruvate simulate the growth of staphylococci. In the ready environment is added a suspension of egg yolk, which gives the opportunity to check lecithinase activity from staphylococci.

Amino acid carboxylase broth

This broth is made in 4 variations: with lysine, ornithine, arginine and the control broth without amino acids. Broth uses for differentiating microorganisms by the possibility of decarboxylating a particular amino acid.

The environment indicator is bromocresol purple, which changes its color from purple (above a pH of 6.8) to yellow (below pH of 5.2). The composition of the medium includes glucose, after its fermentation, acid products are formed, through which the medium turns yellow. After carbon starts amino acids decarboxylate, formed as a result of alkaline products that increase the pH and changing the color of the medium to purple - this indicates a positive reaction for the decarboxylation. If the medium remains yellow, then the microorganisms do not decarboxylate amino acid, which is part of the environment.

Table №1. With lysine

Table №2. With ornithine

Table №3. With arginine

Table №4. Control